Extraction and Separation of Neutral Milk Lipids by Thin-Layer Chromatography

Preparative scale separation of neutral lipids and phospholipids by centrifugally accelerated thin-layer chromatography.

Mixtures of lipids and phospholipids were separated by centrifugally accelerated thin-layer chromatography on a preparative scale (300-500 mg lipid mixture per run). The isolated lipids and phospholipids were identified by 1H and 13C NMR spectroscopy and their fatty acid composition was determined by GLC and GLC-MS of their methyl esters.

Separation of plasma lipids by thin-layer chromatography.

A rapid comprehensive thin-layer chromatographic separation of the major plasma lipid classes on commercial silicic acid glass fiber paper is described. For screening purposes, oxidized fractions are rapidly visualized for qualitative interpretation. Standard colorimetric procedures for each lipid class are per­ formed upon the migration areas cut from the strips. This provides quantitative mea...

Thin-layer Chromatography of Lipids

There has been a tendency to discount thin-layer chromatography (TLC) in recent years because of the availability of high-performance liquid chromatography (HPLC) methods that appeared to offer greater versatility and ease of quantification, amongst other reasons. However, TLC has made a comeback, largely because of the high reproducibility and lower costs of commercial pre-coated plates. It is...

Thin-layer chromatography of tissue lipids without extraction.

Frozen tissue sections were applied directly to silica gel plates, and the lipids were separated by developing the plates in different solvent systems. Quantitation of the lipid classes was achieved by direct transmission densitometry after the plates were treated with a chromic acid-sulfuric acid spray.

Separation of Cholesterol and Demosterol by Thin-layer Chromatography.